Replacing the role of Mrs. Steve on the show as the new next-door neghbor for seasons 2-5, Mrs. Rene was much more friendly and personable.
The Christmas special reveals that she is Jewish and celebrates Hanukkah, but is very tolerant of Pee-wee's religious beliefs as she says "Merry Christmas!" to Pee-wee before he leaves with Santa.
She is played by Suzanne Kent from the second to fifth season of the series.
Mrs. Rene is a plus-sized woman with long orange hair. She has many outfits including her paisley jumper and yellow dress.